Question: RNA-seq data normalisation with housekeeping genes
gravatar for k.ip
16 months ago by
k.ip10 wrote:

Hi there,

I just generated a couple of RNA-seq dataset hoping to identify the differentially expressed genes caused by a particular treatment, for instance, 4 weeks of High fat diet treatment.

So I have used the standard procedures to analyze the data such as TopHat, cufflinks, and cuffdiff to identify the DE gene lists, however, what I am unsure about is that do we need to normalize each biological replicate with some housekeeping genes? Just like RT-qPCR? If so, how do we do that in Galaxy?

Thank you so much!

Cheers, Ken

rna-seq galaxy normalization • 826 views
ADD COMMENTlink written 16 months ago by k.ip10
Please log in to add an answer.


Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 16.09
Traffic: 169 users visited in the last hour