Question: RNA-seq data normalisation with housekeeping genes
gravatar for k.ip
10 months ago by
k.ip10 wrote:

Hi there,

I just generated a couple of RNA-seq dataset hoping to identify the differentially expressed genes caused by a particular treatment, for instance, 4 weeks of High fat diet treatment.

So I have used the standard procedures to analyze the data such as TopHat, cufflinks, and cuffdiff to identify the DE gene lists, however, what I am unsure about is that do we need to normalize each biological replicate with some housekeeping genes? Just like RT-qPCR? If so, how do we do that in Galaxy?

Thank you so much!

Cheers, Ken

rna-seq galaxy normalization • 564 views
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