Question: FTP upload not working in Galaxy
gravatar for parvathi
9 months ago by
parvathi0 wrote:


I was trying upload files to Galaxy via ftp transfer, I am not able to connect it. From yesterday onward this is the case. I was using FileZilla to upload and the message was:

Status: Logged in

Status: Retrieving directory listing...

Command: PWD

Response: 257 "/" is the current directory

Command: TYPE I

Response: 200 Type set to I

Command: PASV

Response: 227 Entering Passive Mode (129,114,60,56,117,87).

Command: MLSD

Error: Connection timed out after 20 seconds of inactivity

Error: Failed to retrieve directory listing

Please help me on this

software error galaxy • 316 views
ADD COMMENTlink modified 9 months ago by Jennifer Hillman Jackson24k • written 9 months ago by parvathi0
gravatar for Jennifer Hillman Jackson
9 months ago by
United States
Jennifer Hillman Jackson24k wrote:


You may have hit the server when it was busy. Please try again now if you have not already. I just FTP uploaded a file to and it was successful.

Some changes to FTP were made on the 19th. It doesn't sound like you are having issues related to the new FTPS protocols (and no issues are known), but I'm going to link that in here anyway for reference:

Thanks, Jen, Galaxy team

ADD COMMENTlink written 9 months ago by Jennifer Hillman Jackson24k

Hi Jen:

I think the new setting of FTP may be inconvenient to some users, could you please keep "the old method" to connect to Galaxy FTP server? Your assistance in this matter is deeply appreciated!!

ADD REPLYlink written 8 months ago by auradunbine20

Update for clarification:

Using the original method of leaving settings in passive mode, if all others settings are left at default, works for most users. The connection is still implemented to be secure. The only change is that when the connection starts up the pop-up window for the security certificate needs to be accepted.


ADD REPLYlink written 4 months ago by Jennifer Hillman Jackson24k
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