Question: TSS distrubtion for ChIP-Seq
0
gravatar for xiang-jiao.yang
5 months ago by
xiang-jiao.yang10 wrote:

I have been using ChIP-Seq and want to display peak distribution at the transcription start sites (TSS). Is there a MakeTSSDist tool in Galaxy main?

chip-seq • 198 views
ADD COMMENTlink modified 4 months ago • written 5 months ago by xiang-jiao.yang10
2
gravatar for Devon Ryan
5 months ago by
Devon Ryan1.8k
Germany
Devon Ryan1.8k wrote:

It makes much more sense to show the signal distribution around the TSS, since peak distribution will rely heavily on the peak caller and its settings. The normal way to do this is to make bigWig files with bamCoverage or bamCompare, compute the coverage around the TSS with computeMatrix reference-point and then plot it with plotProfile or plotHeatmap.

ADD COMMENTlink written 5 months ago by Devon Ryan1.8k
0
gravatar for xiang-jiao.yang
4 months ago by
xiang-jiao.yang10 wrote:

Thanks. It is very helpful.

ADD COMMENTlink written 4 months ago by xiang-jiao.yang10
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