Question: Help! I'm using trimmomatic in Galaxy, but it doesn't recognize a part of input dataset.
gravatar for redgreen
9 months ago by
redgreen10 wrote:

I'm using Trimmomatic to trimming paired end reads. When I set "Single-end or paired-end reads?" to Paired-end (two separate input files), it recognizes files only for Input FASTQ file (R2/second of pair). Therefor, I can choose any fastq files for R2/second pair, on the other hand I could not choose no files for R1/first pair. How do I do to fix the problem??

rna-seq software error • 393 views
ADD COMMENTlink modified 9 months ago by Jennifer Hillman Jackson24k • written 9 months ago by redgreen10

My fastq files are paired end by they were already combined into one. Under trimmomatic, how do I input the fastq files?

ADD REPLYlink written 9 months ago by serrano.gus0
gravatar for Jennifer Hillman Jackson
9 months ago by
United States
Jennifer Hillman Jackson24k wrote:


The dataset "datatype" probably needs to be both confirmed to the in fastqsanger format then have that assigned.


Thanks, Jen, Galaxy team

ADD COMMENTlink written 9 months ago by Jennifer Hillman Jackson24k
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