Question: iGenomes UCSC hg38 annotation gtf file
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mk14609 • 0 wrote:
Dear community,
I have been analysing rna-seq data using Tuxedo on galaxy. I download the iGenomes UCSC hg38 reference annotation .tar.gz file (14.9GB). Extracted the folder onto my computer and followed the path: Homo_sapiens_UCSC_hg38\Homo_sapiens\UCSC\hg38\Annotation\Archives\archive-2015-08-14-08-18-15 Here there are 2 folders (Genes and Genes.gencode) both with a genes.gtf file (148Mb file in genes folder and a 1.333Gb in the Genes.gencode file). And now I am uncertain as to which one to use. Using either as the annotation file through the rna-seq analysis gives slightly differing DEGs and I don't know which is a better representation of my results. If anyone could help it would be very much appreciated!
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modified 20 months ago
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Jennifer Hillman Jackson ♦ 25k
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20 months ago by
mk14609 • 0
