Question: Any One please provide protocol for Analysing long noncoding RNA illumina data in galaxy?
gravatar for shashikanth20
2.3 years ago by
shashikanth2010 wrote:

Hi every one i need assistance on accurate complete protocol on analyzing long non coding RNA illumina data in galaxy. starting from QC parameters to DE analysis

Thank you

noncodingrna • 568 views
ADD COMMENTlink modified 2.3 years ago by Mo Heydarian830 • written 2.3 years ago by shashikanth2010
gravatar for Mo Heydarian
2.3 years ago by
Mo Heydarian830
United States
Mo Heydarian830 wrote:


There are quite a few published workflows available for RNAseq under Shared data --> Workflows on:

Among these workflows is this reference based transcriptome profiling one ( that has an extensive tutorial associated with it (

You can import any of the published workflows to your personal Galaxy account and modify the parameters (and even the tools used) in the workflow editor.

After you have completed your differential expression analysis you can look specifically at your long non-coding RNAs. I would recommend using a reference transcriptome that has annotations for protein-coding and non-coding transcripts, such as Ensembl or RefSeq.


Mo Heydarian, Galaxy Team

ADD COMMENTlink written 2.3 years ago by Mo Heydarian830
Please log in to add an answer.


Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 16.09
Traffic: 180 users visited in the last hour