Today was my first time to present a workshop. Was about RNAseq and i used the webtool Galaxy a did the most common workflow for this type of data (Tophat-Cufflinks). I needed to share the fastq files with several students so i used the option of publish the data (public) that i had in one of my histories. I tested the workflow and everything went well... but during the class exactly on first step of retrieve data i got an error that i never saw it! The students couldnt drag the files from my history (that was previous imported to their own history) manipulate, download or even view. I dont remember the error but was something about the permissions and admin... The "funny thing" was after passing a few (long) minutes everything was fine and they could perform the workflow!
I am trying to figure out why this happened ... Is it because the internet connection? IT permissions (here in my institute we have very restrict rules using the internet, server, student computers...)? Need I to be online to perform correctly the share of history?
Another thing, one student lost the process (output files) of workflow that he did... i already read about this here in the forum so i am asking if the Galaxy is so instable. Normally i perform the workflows using command line but was a workshop for people that don know how to use linux... so thats why i used galaxy.
Beside that i think galaxy its very cool for testing with small samples and try to figure out what are the best NGS tools to use.
Best Regards, Andreia