Question: zero fpkm value in cufflink
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gravatar for 1603.neha
2.6 years ago by
1603.neha70
1603.neha70 wrote:

hi i have done cufflink on my sra seq according to the ngs pipeline. the result says fpkm value zero and fpkm status ok.. what does it mean? what is the error in my performing the job?

rna-seq cufflinks fpkm • 1.0k views
ADD COMMENTlink modified 2.6 years ago by yena.oh70 • written 2.6 years ago by 1603.neha70
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gravatar for yena.oh
2.6 years ago by
yena.oh70
Canada
yena.oh70 wrote:

Hi,

From your other post, it seems like there is an error with your reads alignment (tophat) step, as you had 0% of the input reads that were aligned to the reference genome, which would lead to fpkms of zero.

Check your other post for suggestions: cuffdiff error and time

Good luck!

Yena

ADD COMMENTlink written 2.6 years ago by yena.oh70
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