3.4 years ago by
Use a yeast genome as a Custom Reference Genome. UCSC is one source. Genomes at this site are in the downloads area inside directories named "Full data set": http://hgdownload.soe.ucsc.edu/downloads.html#yeast
Download the target file using UCSC's instructions, uncompress if tar.gz, then load into Galaxy via the upload tool. The 2bit version is also an option if you have the UCSC source tools on your computer (convert using twoBitToFasta). However, in most cases, the file you want will be named something like "chromFa.tar.gz". If the genome is split by chromosome, merge these together on your computer before loading into Galaxy with line-command tools, or merge in Galaxy after loading each with the tool "Concatenate".
Best, Jen, Galaxy team