To do this within Galaxy, use a local or cloud and this tool shed repository:
If you want to do this line-command, the legacy tools can be obtained here. 32-bit is no longer supported, but seems to be available.
Best, Jen, Galaxy team
Thank you very much for the reply. I have installed both SRAtoolkit and NGSFC . They are able to convert sra files to fastaq but those fastaq files are not giving me FASTA QC results in galaxy. Therefore, to confirm it I obtained a fastaq file from outside. And that fastaq file was perfectly opening in galaxy and gave me FASTA QC results. But all the fastaq files I made in my PC with NGSFC is not giving me FASTA QC results in galaxy. so what should I do now? it always shows red color error with my FASTAq files.