Question: DO I need tags for primer optimization?
gravatar for Rilla
3.8 years ago by
Rilla0 wrote:

Hi All, does anyone have good advice on using universal tags when testing newly designed primers? I've read that adding a tag (M13 or CAG) reduces artifacts during the PCR process so the products are clearer for scoring on an agarose gel during the initial amplification  and optimization trials. Is this correct? Should I add a  tag to my primers (NGS and designed using combo of QDD and Msat Commander)?

cag m13 ngs primer design tag • 729 views
ADD COMMENTlink modified 3.8 years ago by Jennifer Hillman Jackson25k • written 3.8 years ago by Rilla0
gravatar for Jennifer Hillman Jackson
3.8 years ago by
United States
Jennifer Hillman Jackson25k wrote:


This is probably not the best forum for detailed wet-lab protocol feedback. The primary Biostars may also not be a good fit. Perhaps try if you have not already?

That said, once you decide what to do, there is a Galaxy wrapper for QDD in the tool shed (for use in a local or cloud Galaxy).

Thanks, Jen, Galaxy team

ADD COMMENTlink written 3.8 years ago by Jennifer Hillman Jackson25k
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