Hi All, does anyone have good advice on using universal tags when testing newly designed primers? I've read that adding a tag (M13 or CAG) reduces artifacts during the PCR process so the products are clearer for scoring on an agarose gel during the initial amplification and optimization trials. Is this correct? Should I add a tag to my primers (NGS and designed using combo of QDD and Msat Commander)?
This is probably not the best forum for detailed wet-lab protocol feedback. The primary Biostars may also not be a good fit. Perhaps try seqanswers.com if you have not already?
That said, once you decide what to do, there is a Galaxy wrapper for QDD in the tool shed (for use in a local or cloud Galaxy).
Thanks, Jen, Galaxy team