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Question: DO I need tags for primer optimization?
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gravatar for Rilla
3.8 years ago by
Rilla • 0
Rilla • 0 wrote:

Hi All, does anyone have good advice on using universal tags when testing newly designed primers? I've read that adding a tag (M13 or CAG) reduces artifacts during the PCR process so the products are clearer for scoring on an agarose gel during the initial amplification  and optimization trials. Is this correct? Should I add a  tag to my primers (NGS and designed using combo of QDD and Msat Commander)?
cag m13 ngs primer design tag • 729 views
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modified 3.8 years ago by Jennifer Hillman Jackson ♦ 25k • written 3.8 years ago by Rilla • 0
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gravatar for Jennifer Hillman Jackson
3.8 years ago by
Jennifer Hillman Jackson ♦ 25k
United States
Jennifer Hillman Jackson ♦ 25k wrote:

Hello, 

This is probably not the best forum for detailed wet-lab protocol feedback. The primary Biostars may also not be a good fit. Perhaps try seqanswers.com if you have not already?

That said, once you decide what to do, there is a Galaxy wrapper for QDD in the tool shed (for use in a local or cloud Galaxy).
http://usegalaxy.org/toolshed

Thanks, Jen, Galaxy team
ADD COMMENT • link written 3.8 years ago by Jennifer Hillman Jackson ♦ 25k
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