Question: MACS for ChIP seq of histone modifications in S. cerevisiae
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Law, Michael J. • 20 wrote:
I am performing ChIP-seq of histone modifications in S. cerevisiae. The trouble occurs when I am using MACS. If I use the correct genome size of 1.2e+7 and almost any value of MFOLD (as low as 3), I don't get any peaks. If the only parameter I change is the genome size to 2.7e+9, I get peaks, albeit they are not correct.
Since it is histone mods and not TF binding, is MACS even appropriate to use? Would SICER be a better choice?
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modified 4.4 years ago
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Jennifer Hillman Jackson ♦ 25k
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4.4 years ago by
Law, Michael J. • 20