Question: How To Define The Cutoff Value Of Rpkm For Expressed Genes?
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gravatar for Hoang, Thanh
5.4 years ago by
Hoang, Thanh200
Hoang, Thanh200 wrote:
Hi all, I have been working on RNA-seq data analysis using TopHat and Cuffdiff. One of the problem I have is to define the cutoff RPKM value to tell whether a gene is expressed from the background noise?. Could anybody give me a suggestion? Thank you Thanh
rna-seq cuffdiff • 3.2k views
ADD COMMENTlink modified 5.4 years ago by Jeremy Goecks2.2k • written 5.4 years ago by Hoang, Thanh200
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gravatar for Jeremy Goecks
5.4 years ago by
Jeremy Goecks2.2k
Jeremy Goecks2.2k wrote:
The confidence intervals provided by Cufflinks/Cuffdiff are a good place to start; any confidence interval that includes 0 should be looked on skeptically. Good luck, J.
ADD COMMENTlink written 5.4 years ago by Jeremy Goecks2.2k
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