Question: Re: Quality Trimming Of Sequences Before Rna-Seq
gravatar for Bomba Dam
6.8 years ago by
Bomba Dam40
Bomba Dam40 wrote:
Dear Noa, I also feel the same. So I think it would be better to use these three steps remove sequencing artifacts then trim the sequences by from the 5' end or if needed from the 3'end also (2-5 bases, depending on your sequence quality) finally filter the sequences by quality (I used the default parameters). Although it removed 15% of the sequences but I feel confident with the high quality data. Any better suggestion will be appreciated. CHeers, Bomba -- Dr. BOMBA DAM Alexander von Humboldt Postdoctoral Research Fellow Max-Planck-Institut für terrestrische Mikrobiologie Karl-von-Frisch-Straße 10 D-35043 Marburg, Germany E mail: PHONE: +49 176 321 321 75 (Mobile); +49 6421 178 721 (LAB); +49 6421 2828516 (ROOM) Assistant Professor of Microbiology Department of Botany, Institute of Science Visva-Bharati (A Central University) Santiniketan, West Bengal 731235, India. E mail:,;
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