Question: HISAT2 errors with fastq input -- double check input formatting/content
0
gravatar for kdong
6 months ago by
kdong0
kdong0 wrote:

Hi everyone!

I am new in RNA-seq research and try to use galaxy main to analysis my data.

I met the error when running HISAT2, it said my file does not look like FASTQ but my datatype is fastqsanger and the file name is "fastq.gz". I have tried several times but it all looks like this. Please let me know if you have any suggestions!

error report: Error: reads file does not look like a FASTQ file terminate called after throwing an instance of 'int' (ERR): hisat2-align died with signal 6 (ABRT) (core dumped)

Thank you very much! I appreciate!

Duke

ADD COMMENTlink modified 6 months ago by Jennifer Hillman Jackson25k • written 6 months ago by kdong0
0
gravatar for Jennifer Hillman Jackson
6 months ago by
United States
Jennifer Hillman Jackson25k wrote:

Hello,

It looks like you worked out the content or format problems and have successful jobs now.

For others that may run into a similar problem, start troubleshooting by checking that your fastq data was completely uploaded and assigned the proper datatype. Support FAQs: https://galaxyproject.org/support/#troubleshooting

Thanks! Jen, Galaxy team

ADD COMMENTlink written 6 months ago by Jennifer Hillman Jackson25k
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