Question: How to prepare FastQ files for HISAT2
0
gravatar for skhan
9 months ago by
skhan40
skhan40 wrote:

Hi there,

Should I be preparing or otherwise cleaning fastq files before I try to align them to a reference genome using HISAT2? If yes, how and using which tools?

I have 2x 76 bases paired end (separate R1 and R2 files) reads and am attempting RNA-Seq.

Thanks.

fastq alignment rna-seq hisat2 • 657 views
ADD COMMENTlink modified 8 months ago by Jennifer Hillman Jackson25k • written 9 months ago by skhan40
0
gravatar for Jennifer Hillman Jackson
8 months ago by
United States
Jennifer Hillman Jackson25k wrote:

Hello,

Please see the Galaxy tutorials here: https://galaxyproject.org/learn/

Start with these:

  • NGS logistics - this is an introduction to Galaxy's functionality for the analysis of Next Generation Sequencing data.
  • RNA-seq: Introduction - an introductory tutorial for transcriptome analysis.
  • RNA-seq: Discovering and quantifying new transcripts - an in-depth transcriptome analysis example.

Thanks! Jen, Galaxy team

ADD COMMENTlink written 8 months ago by Jennifer Hillman Jackson25k
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 16.09
Traffic: 176 users visited in the last hour