I did uproading file through EBI homepage about NCBI Geo dataset SRR 5682257 &5682258. [EBI SRA: SRX2917412 File: ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR568/007/SRR5682257/SRR5682257.fastq.gz] and it was convert using FASTQ Groomer Tool (convert between various FASTQ quality formats) at NGS: QC and manipulation. because first file is gz file that are compressed file. next, i did click FastQC Tool (Read Quality reports), so that i see Quality control of this dataset.
unfortunately, per tile sequence quality and sequence Duplication levels got X. i am user who use Galaxy tool first time for analysis NCBi dataset. so i am poor.
In this case, Is this data sets already finished Trimming by original uproader?? so am i just have to start analysis alingment using Bowtie ? other wise, IS this dataset' Quality very low?
what i have got problem T-T... please help me.