I am working with a GFF file, which is missing 5' UTR annotations. I thought I might be able to add UTR data using the tools on Galaxy as follows:
1) Convert the existing GFF file to Excel format (.xls) using the pencil icon on Galaxy. 2) Download the Excel file and make changes. 3) Upload the modified Excel file and convert it back to GFF using the pencil icon tool. 4) Run HtSeq on RNA-Seq data and the new GFF file to get counts corresponding to each UTR.
However, when I did this the I got no data output. Can anyone suggest what I might have done wrong or how I can modify GFF files using simple tools that do not require bioinformatics expertise?