Question: How to use cuffmerge and cuffdiff
0
gravatar for lrm_1985
9 weeks ago by
lrm_19850
lrm_19850 wrote:

I am trying use Galaxy to analysis the yeast RNA-seq data. When I want to try the cuffmerge and cuffdiff, I have questions. For the cuffmerge, should I merge the repeat of the samples or different genotype strain? Base on this questions, how should I do when I run the the cuffdiff using the results from cuffmerge?

rna-seq • 112 views
ADD COMMENTlink modified 9 weeks ago by Jennifer Hillman Jackson22k • written 9 weeks ago by lrm_19850
0
gravatar for Jennifer Hillman Jackson
9 weeks ago by
United States
Jennifer Hillman Jackson22k wrote:

Hello,

In short, use Cuffmerge to create a "master" GTF dataset, with the input as the result GTF datasets from Cufflinks (all produced in the experiment) plus the reference annotation GTF dataset (if one is to be used).

The result from Cuffmerge is the reference annotation GTF input to Cuffdiff. The other inputs will be the mapped BAM datasets, with replicates (1 or more) grouped by condition.

Manual and tutorial examples:

Thanks! Jen, Galaxy team

ADD COMMENTlink written 9 weeks ago by Jennifer Hillman Jackson22k

Thanks, I will read the examples. Hope I can figure out soon

ADD REPLYlink written 9 weeks ago by lrm_19850
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