In my local galaxy , I used Tool Shed TopHat Gapped-read mapper for RNA-seq data (Galaxy Version 2.1.1)，they need Dependency Resolver Details :
Dependency Version Resolver Exact version Current Installation Status
bowtie2 2.2.8 Galaxy_Package True
tophat 2.1.1 Galaxy_Package True
I prepared，and the following is the wrong question
<h6>################ By Message</h6>Fatal error: Tool execution failed Building a SMALL index
[2017-02-16 15:06:47] Beginning TopHat run (v2.1.1)
[2017-02-16 15:06:47] Checking for Bowtie Bowtie version: 2.2.8.0 [2017-02-16 15:06:47] Checking for Bowtie index files (genome).. [2017-02-16 15:06:47] Checking for reference FASTA file [2017-02-16 15:06:47] Generating SAM header for genome [2017-02-16 15:06:47] Preparing reads left reads: min. length=75, max. length=75, 100 kept reads (0 discarded) right reads: min. length=75, max. length=75, 100 kept reads (0 discarded) [2017-02-16 15:06:47] Mapping left_kept_reads to genome genome with Bowtie2 [2017-02-16 15:06:47] Mapping left_kept_reads_seg1 to genome genome with Bowtie2 (1/3) [2017-02-16 15:06:47] Mapping left_kept_reads_seg2 to genome genome with Bowtie2 (2/3) [2017-02-16 15:06:47] Mapping left_kept_reads_seg3 to genome genome with Bowtie2 (3/3) [2017-02-16 15:06:47] Mapping right_kept_reads to genome genome with Bowtie2 [2017-02-16 15:06:47] Mapping right_kept_reads_seg1 to genome genome with Bowtie2 (1/3) [2017-02-16 15:06:48] Mapping right_kept_reads_seg2 to genome genome with Bowtie2 (2/3) [2017-02-16 15:06:48] Mapping right_kept_reads_seg3 to genome genome with Bowtie2 (3/3) [2017-02-16 15:06:48] Searching for junctions via segment mapping [FAILED] Error: segment-based junction search failed with err =-5 Reason: image not found
can someone help me this question ? Appreciate !!! :) by ru-feng
