Question: TopHat with Culex quinquefasciatus genome
0
gravatar for cheolho_sim
19 days ago by
cheolho_sim0 wrote:

I try to use TopHat for RNA-seq data. The Culex quinquefasciatus genome will be used for the TopHat. Would you me a guide which genome data that I need to use along with RNA-seq data (from Illumina HighSeq)?

Here is the Culex quinquefasciatus genome data webpage. https://www.vectorbase.org/organisms/culex-quinquefasciatus

Assembly, Gene set, Scaffolds?

rna-seq • 49 views
ADD COMMENTlink modified 18 days ago by Jennifer Hillman Jackson21k • written 19 days ago by cheolho_sim0
0
gravatar for Jennifer Hillman Jackson
18 days ago by
United States
Jennifer Hillman Jackson21k wrote:

Hello,

Use the Scaffolds data as the target reference genome.

Load the fasta file into Galaxy and use it as a Custom reference genome with Tophat and other tools. https://wiki.galaxyproject.org/Support#Custom_reference_genome

Thanks, Jen, Galaxy team

ADD COMMENTlink written 18 days ago by Jennifer Hillman Jackson21k
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 16.09
Traffic: 58 users visited in the last hour