Question: Binding strength of chromosome regions
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gravatar for cgard2
24 months ago by
cgard2 • 10
cgard2 • 10 wrote:

How do you determine the strength of binding site of a chromosome region? We need to report top 10 regions in chromosome of strongest binding.

chip-seq • 531 views
ADD COMMENT • link • modified 23 months ago by Jennifer Hillman Jackson ♦ 25k • written 24 months ago by cgard2 • 10
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gravatar for Jennifer Hillman Jackson
23 months ago by
United States
Jennifer Hillman Jackson ♦ 25k wrote:

Hello,

I am not sure what tool you are using, but once peaks are called the output can be filtered/compared to determine those with the most coverage/depth. See the tools in the group NGS: Peak Calling at Galaxy Main (http://usegalaxy.org) if you have not chosen one yet. Review the tool forms and 3rd party documentation to understand the output metrics and what they represent.

If you are looking for a tutorial and/or examples for how to perform the analysis, googling "galaxy chip-seq" brings up many learning options.

Thanks, Jen, Galaxy team

ADD COMMENT • link written 23 months ago by Jennifer Hillman Jackson ♦ 25k
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