I did RNA sequencing and many reads map to tRNA regions. Specific tRNAs are completely covered by all the reads that map to it, but many reads are not full-length tRNA. (So some reads start at 5'end while others start for example at base 20) To find out to which part of the tRNA reads correspond, I would like to sort (and count) reads by their start position. (I already used the tool intersect to determine fraction of tRNA overlap). Does anyone know which tool from the Galaxy platform I can use to do this??
Thanks in advance. Carla