HI, I am running local instance of Galaxy on my linux mint system. When I try to run CuffDiff, I am getting the following error (see below) When I look in the folder "/home/vythegi/galaxy1111/database/jobs_directory/000/" I cannot find a folder named (150" or tool_script.sh. I would really appreciate it if you can help me sort this out. Thanks in advance for your time.
Fatal error: Exit code 127 () [09:23:24] Loading reference annotation. Warning: No conditions are replicated, switching to 'blind' dispersion method [09:23:25] Inspecting maps and determining fragment length distributions. [09:26:02] Modeling fragment count overdispersion. Map Properties: Normalized Map Mass: 6915184.62 Raw Map Mass: 6698481.22 Fragment Length Distribution: Empirical (learned) Estimated Mean: 179.51 Estimated Std Dev: 60.34 Map Properties: Normalized Map Mass: 6915184.62 Raw Map Mass: 7110254.01 Fragment Length Distribution: Empirical (learned) Estimated Mean: 186.65 Estimated Std Dev: 62.64 [09:26:51] Calculating preliminary abundance estimates [09:26:51] Testing for differential expression and regulation in locus. Processed 6432 loci.
Performed 5391 isoform-level transcription difference tests Performed 5209 tss-level transcription difference tests Performed 5148 gene-level transcription difference tests Performed 0 CDS-level transcription difference tests Performed 0 splicing tests Performed 0 promoter preference tests Performing 0 relative CDS output tests Writing isoform-level FPKM tracking Writing TSS group-level FPKM tracking Writing gene-level FPKM tracking Writing CDS-level FPKM tracking Writing isoform-level count tracking Writing TSS group-level count tracking Writing gene-level count tracking Writing CDS-level count tracking Writing isoform-level read group tracking Writing TSS group-level read group tracking Writing gene-level read group tracking Writing CDS-level read group tracking Writing read group info Writing run info /home/vythegi/galaxy1111/database/jobs_directory/000/150/tool_script.sh: line 9: R: command not found"