I'm going through the RNAseq tutorial posted here:
Data upload and setting up collection goes well; however, I run into a problem with the tophat step. I keep getting a "Tool Error: An error with this dataset" I am using the dataset that I downloaded from the tutorial, so not sure what the issue will be.
Here is the detailed error I received: "Traceback (most recent call last): File "/home/vythegi/galaxy/lib/galaxy/jobs/runners/local.py", line 128, in queue_job job_wrapper.finish( stdout, stderr, exit_code ) File "/home/vythegi/galaxy/lib/galaxy/jobs/__init__.py", line 1265, in finish dataset.datatype.set_meta( dataset, overwrite=False ) File "/home/vythegi/galaxy/lib/galaxy/datatypes/binary.py", line 347, in set_meta exit_code = subprocess.call( args=command, stderr=open( stderr_name, 'wb' ) ) File "/usr/lib/python2.7/subprocess.py", line 523, in call return Popen(popenargs, *kwargs).wait() File "/usr/lib/python2.7/subprocess.py", line 711, in __init__ errread, errwrite) File "/usr/lib/python2.7/subprocess.py", line 1340, in _execute_child raise child_exception OSError: [Errno 2] No such file or directory"
Any help you can provide is greatly appreciated.