Question: top hat splice junction
0
gravatar for 1603.neha
2.3 years ago by
1603.neha70
1603.neha70 wrote:

hi i am working on ngs data and used tophat for aligning the sra data to refernce genome. top hat output file is splice junction. i want to know that how to analyse it as it gives a table. like if i want to calculate no. of splice junctions.how to do it.please help. thanks

rna-seq tophat • 776 views
ADD COMMENTlink modified 2.3 years ago • written 2.3 years ago by 1603.neha70
0
gravatar for Jennifer Hillman Jackson
2.3 years ago by
United States
Jennifer Hillman Jackson25k wrote:

Hello,

The number of lines in this output dataset is the number of junctions. The data can be manipulated further with tools that accept BED or tabular input.

More details: Tophat Output Spice Junction Output

Best, Jen, Galaxy team

ADD COMMENTlink written 2.3 years ago by Jennifer Hillman Jackson25k
0
gravatar for 1603.neha
2.3 years ago by
1603.neha70
1603.neha70 wrote:

hi thanks a lot jen

ADD COMMENTlink written 2.3 years ago by 1603.neha70
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