Question: Adapter trimming in basespace
gravatar for shamsher jagat
2.5 years ago by
United States
shamsher jagat590 wrote:

I have used NuGEN RNA-seq kit and adapter sequence. is AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT (TruSeq Universal Adapter) However in base space (FASTqToolkit) closest one is True seq dual HT/LT but it is not completely matching Another option is Ht/Lt common seq of 12 bases. What should be my best choice. In base space I cannot paste my adaptor seq.



ADD COMMENTlink modified 2.5 years ago by Jennifer Hillman Jackson25k • written 2.5 years ago by shamsher jagat590
gravatar for Jennifer Hillman Jackson
2.5 years ago by
United States
Jennifer Hillman Jackson25k wrote:


For help with that application, it would be best to contact Illumina support. Or you can try posting to a general bioinformatics board such as

If you want to instead process the data in Galaxy (, there are other trimming tools available. At, these are in the tool group NGS: QC and manipulation. In a local or cloud Galaxy, find tools in the Tool Shed ( and install for use.

Thanks, Jen, Galaxy team

ADD COMMENTlink written 2.5 years ago by Jennifer Hillman Jackson25k
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