Question: Comparing two datasets with a custom genome
0
gravatar for lhorb
3.2 years ago by
lhorb0
United States
lhorb0 wrote:

I have an ran seq experiment -paired end, 100 bp, where I am trying to compare control vs test samples.  I am using a Xenopus Laevis genome (Xenbase v9.1)  which I uploaded via FTP.   I uploaded the data sets, trimmed them and then aligned them with TOPHAT tool to my 'custom genome from history'.

When I took the course, at this point we looked at the data but we were using a human genome that was available and there was a link in the TOPHAT accepted hits to USCU main.  My genome is not there so there is no such link and now I'm lost.    I would just like to compare the data sets to see if there is anything in the test which is not in the control.  Thank you

 

 

 

 

rna-seq ucsc customgenome • 878 views
ADD COMMENTlink modified 3.1 years ago by Jennifer Hillman Jackson25k • written 3.2 years ago by lhorb0
0
gravatar for Jennifer Hillman Jackson
3.2 years ago by
United States
Jennifer Hillman Jackson25k wrote:

Hello,

Create a Custom Build and use Trackster within Galaxy for visualization. 

https://wiki.galaxyproject.org/Learn/CustomGenomes#Custom_Builds
https://wiki.galaxyproject.org/Learn/Visualization#Custom_Builds

Thanks! Jen, Galaxy team

 

ADD COMMENTlink written 3.2 years ago by Jennifer Hillman Jackson25k
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