(sorry for my bad english), I would use bwa-mem in Galaxy to map sequences against human genome. I have the file fast.q of Roche454 Junior, in this there is not specified if the sequence is in forward or in reverse. In this case, what is the option which allows to use BWA-MEM?
I have another question, I have installed Galaxy in local, but when I try to install BWA-MEM, I see this error in package_bwa: "Installed, missing tool dependencies", in particular:
Error downloading from URL
HTTP Error 403: Forbidden: category denied
I think that the administration where I work denied automatically the download. How to fix it?
I really hope someone can help me.
Thank you in advance.