I am new to galaxy. I wish to use the Extract Genomic DNA tool, but under "Fetch sequences for intervals in" it says "No interval or gff dataset available." In my history, I have a tabular interval dataset generated by Pileup-to-Interval, which was created via Generate Pileup from BAM. The build is specified under attributes for this dataset.
Any idea why the tool is not recognizing this interval dataset?
My end goal is to get fasta sequences for small (gene-size) genomic intervals for a strain (bam file) mapped to the reference genome. Specifically, I was following Jennifer Hillman-Jackson's suggestion on this query: https://www.biostars.org/p/1388/.
Thank you for any assistance you can offer!