Question: trying to do MACS!!!
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tali.mandel • 10 wrote:
Hello,
I have a file in BAM format of chip-seq I did in galaxy
when i try to do MACS on it I get this error message that I have no idea what it means:
INFO @ Wed, 19 Nov 2014 04:40:52: # ARGUMENTS LIST: # name = MACS_in_Galaxy # format = BAM # ChIP-seq file = /galaxy-repl/main/files/009/715/dataset_9715201.dat # control file = None # effective genome size = 9.00e+07 # tag size = 25 # band width = 300 # model fold = 6 # pvalue cutoff = 1.00e-05 # Ranges for calculating regional lambda are : peak_region,1000,5000,10000 INFO @ Wed, 19 Nov 2014 04:40:52: #1 read tag files... INFO @ Wed, 19 Nov 2014 04:40:52: #1 read treatment tags... INFO @ Wed, 19 Nov 2014 04:41:02: 1000000 INFO @ Wed, 19 Nov 2014 04:41:12: 2000000 INFO @ Wed, 19 Nov 2014 04:41:22: 3000000 INFO @ Wed, 19 Nov 2014 04:41:35: 4000000 INFO @ Wed, 19 Nov 2014 04:41:45: 5000000 INFO @ Wed, 19 Nov 2014 04:41:55: 6000000 INFO @ Wed, 19 Nov 2014 04:42:05: 7000000 INFO @ Wed, 19 Nov 2014 04:42:15: 8000000 INFO @ Wed, 19 Nov 2014 04:42:25: 9000000 INFO @ Wed, 19 Nov 2014 04:42:35: 10000000 INFO @ Wed, 19 Nov 2014 04:42:44: 11000000 INFO @ Wed, 19 Nov 2014 04:42:54: 12000000 INFO @ Wed, 19 Nov 2014 04:43:04: 13000000 INFO @ Wed, 19 Nov 2014 04:43:14: 14000000 INFO @ Wed, 19 Nov 2014 04:43:22: 15000000 INFO @ Wed, 19 Nov 2014 04:43:30: 16000000 INFO @ Wed, 19 Nov 2014 04:43:38: 17000000 INFO @ Wed, 19 Nov 2014 04:43:46: 18000000 INFO @ Wed, 19 Nov 2014 04:43:54: 19000000 INFO @ Wed, 19 Nov 2014 04:44:01: 20000000 INFO @ Wed, 19 Nov 2014 04:44:09: 21000000 INFO @ Wed, 19 Nov 2014 04:44:17: 22000000 INFO @ Wed, 19 Nov 2014 04:44:32: #1 Background Redundant rate: 0.03 INFO @ Wed, 19 Nov 2014 04:44:32: #1 finished! INFO @ Wed, 19 Nov 2014 04:44:32: #2 Build Peak Model... INFO @ Wed, 19 Nov 2014 04:44:37: #2 number of paired peaks: 1 WARNING @ Wed, 19 Nov 2014 04:44:37: Too few paired peaks (1) so I can not build the model! Lower your MFOLD parameter may erase this error. WARNING @ Wed, 19 Nov 2014 04:44:37: Process is terminated!
Can I do MACS on one sample? (with no control?)
Help please
Tali
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modified 4.0 years ago
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Dannon Baker ♦ 3.7k
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4.0 years ago by
tali.mandel • 10
