hello,i am grateful for your answers.
I have two questions,
First,before i do map fastq to fasta,whether i could do bwa index for fasta,or the "mapping with BWA "in tool pane is got ready in the "mapping with BWA" software package.
Second,i can not find "sortsam"in Picard tool pane when i do according procedure"mark duplicate",how can i do next step? whether any other tool could instead of "sortsam",or whether any other workflow i can finish the DNAseq all using tools in web-based Galaxy?