Question: Chip-Seq Normalization To Total Number Of Reads
1
gravatar for Wooi Lim
5.0 years ago by
Wooi Lim30
Wooi Lim30 wrote:
Dear Galaxy, I am trying to analyze my ChIP-Seq data from Illumina using Galaxy. I have 2 datasets that I want to compare after normalizing each of them to their respective inputs, and these 2 datasets have very different number of reads to start with, is there a way to first normalize each dataset to total number of reads in Galaxy? Thanks. Your help is very much appreciated. Catheryn
chip-seq • 2.7k views
ADD COMMENTlink modified 5.0 years ago by Björn Grüning350 • written 5.0 years ago by Wooi Lim30
1
gravatar for Björn Grüning
5.0 years ago by
Björn Grüning350 wrote:
Hi Catheryn, for ChIP-seq analysis, normalisation and BAM file correlation we use deeptTools. Here you can read more about it: https://github.com/fidelram/deepTools And here is the toolshed repository: http://toolshed.g2.bx.psu.edu/view/bgruening/deeptools Cheers, Bjoern
ADD COMMENTlink written 5.0 years ago by Björn Grüning350
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