Question: How Much Fpkm Can Be Take Into Consideration When Compare Gene Expression
0
Du, Jianguang • 380 wrote:
Dear All,
I am comparing the gene expression between two cell types by examining
the Cufflink output file -- gene differential expression
testing<javascript:void(0);>. The file lists the FPKM of genes in two
cell types and log2 of fold. I want to look for genes that have more
than 2-flod of expression in cell type A than in cell type B. What is
the minimum FPKM in cell type A so that only the genes that have FPKM
highier than this number can be taken into consideration for further
analysis?
For example,
The FPKM of gene X in cell type A is 80, and in cell type B is 20, the
fold of difference is 4.
The FPKM of gene Y in cell type A is 4, and in cell type B is 1, the
fold of difference is also 4.
Is there a minimum FPKM in cell type A for genes to be selected for
further analysis?
Thanks.
Jianguang
ADD COMMENT
• link
•
modified 6.2 years ago
by
Luciano Cosme • 220
•
written
6.2 years ago by
Du, Jianguang • 380
