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6.4 years ago by

i b • 330

i b • 330 wrote:

ok, so if I have 3 different samples I would use 3 different goups and add my samples as replicate within each group. or did you mean to create just one group and add all my 3 samples as 3 replicates within that only group? thanks a lot ngs-ib ________________________________________ To: Irene Bassano Cc: galaxy-user@lists.bx.psu.edu Subject: Re: [galaxy-user] cuffdiff Hello Irene, two files Change the tool form for the option "Perform replicate analysis:" to be "Yes" (the default is "No"). Use two conditions (Groups). GTF output from any of these may be appropriate. Please see the tutorial on Galaxy main at: http://main.g2.bx.psu.edu/u/jeremy/p/galaxy-rna-seq-analysis-exercise FAQ: http://main.g2.bx.psu.edu/u/jeremy/p/transcriptome-analysis-faq And the "Common uses" protocols on the Cufflinks web site plus their new paper (also linked at this site, from side bar, as "Protocol"): http://cufflinks.cbcb.umd.edu/tutorial.html Best, Jen Galaxy team -- Jennifer Jackson http://galaxyproject.org

rna-seq cufflinks • 806 views

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modified 6.4 years ago by Jennifer Hillman Jackson ♦ 25k • written 6.4 years ago by i b • 330

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