Requesting Uploading Of Genomes

Question: Requesting Uploading Of Genomes

6.9 years ago by

Hi, Would be grateful I the following genomes were uploaded into Galaxy Streptomyces coelicolor A3(2) ; ftp://ftp.ncbi.nih.gov/genomes/Bacteri a/Streptomyces_coelicolor_A3_2__uid57801/NC_003888.gbk Propionibacterium acnes KPA171202 (pre-salt downshift); ftp://ftp.ncbi .nih.gov/genomes/Bacteria/Propionibacterium_acnes_KPA171202_uid58101/N C_006085.gbk Thanks in advance, Kenneth Dr Kenneth McDowall * Astbury Centre for Structural Molecular Biology Tel +44 (0) 113 343 3109 or Mob. 0783 793 5384 | Ext 33109 | k.j.mcdowall@leeds.ac.uk<mailto:k.j.mcdowall@leeds.ac.uk> | www.fbs.leeds.ac.uk IMCB Director of Taught Programmes * Institute of Molecular and Cellular Biology Faculty of Biological Sciences | University of Leeds | Manton Building Room 10.05 | Leeds LS2 9JT | UK This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. Access, copying, dissemination and re-use of information in it by anyone else are unauthorised. If you have received this message in error, please contact the sender as soon as possible.

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modified 6.9 years ago by Jennifer Hillman Jackson ♦ 25k • written 6.9 years ago by Kenneth McDowall • 10

6.9 years ago by

Jennifer Hillman Jackson ♦ 25k

United States

Jennifer Hillman Jackson ♦ 25k wrote:

Hello Kenneth, Most tools that use a reference genome will accept a custom reference genome, including the visualization tool to create a custom browser. The recommendation is to: 1 - load the genome in FASTA format into your working history using FTP http://wiki.g2.bx.psu.edu/Learn/Upload%20via%20FTP 2 - on the target tool form, use the custom reference genome option (aka genome "from the history") Best practice is to use the same custom reference genome dataset for all analysis steps to avoid format/identifier problems. If annotation files (GTF, etc.) are incorporated, be certain to double check that the chromosome/scaffold identifiers are an exact match, and modify if necessary, before use. Please let us know if you would like more help, Best, Jen Galaxy team -- Jennifer Jackson http://usegalaxy.org http://galaxyproject.org/wiki/Support

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