Question: Bed12 Format For Stitching Blocks Given A Set Of Coding Exon Intervals
gravatar for Amit Indap
7.1 years ago by
Amit Indap60
Amit Indap60 wrote:
Hi Galaxy, I am trying to stitch together MAF alignments for the coding sequence for a few genes of interest in Drosophila. I used UCSC to send the bed intervals of the coding exons of my gene and sent the output to Galaxy. But when I try and use the tool "Stitch Gene blocks" it complains that my bed is a bed3 and not a bed12. I'm a bit rusty with my browser skills, but how can I send my output to Galaxy as a bed12 format so I can stitch my MAF blocks together? Thanks for your help! Amit -- Amit Indap
galaxy • 1.2k views
ADD COMMENTlink modified 7.1 years ago by Daniel Blankenberg ♦♦ 1.7k • written 7.1 years ago by Amit Indap60
gravatar for Daniel Blankenberg
7.1 years ago by
Daniel Blankenberg ♦♦ 1.7k
United States
Daniel Blankenberg ♦♦ 1.7k wrote:
Hi Amit, Without taking a look at your history, I'll have to make a guess. When you retrieve regions from UCSC, on the second step, right before you click "Send query to Galaxy", make sure that you have "Whole Gene" selected under "One record per", and that you are looking at a gene track and that the format was set to "BED" on the first page. Also be sure to Not include a track header. Thanks for using Galaxy, Dan
ADD COMMENTlink written 7.1 years ago by Daniel Blankenberg ♦♦ 1.7k
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