That annotation is a good match for hg38. Be sure to use the GTF version of the annotation. There are three choices and which to use depends on what you are doing -- for many, the CHR version is the easiest to work with (simpler). You could run the analysis using the different version and compare to make the decision for yourself.
The data will load with the datatype
gff assigned due to the presence of header lines. Some tools can use the data that way, others will require that you remove the header lines and change the datatype to be
Remove header lines with the tool Select using the options "NOT Matching" and the regular expression
Thanks! Jen, Galaxy team
Thank you, that helps out a lot. I used the GTF version that was provided in the link to the right of all the files. Does this resolve those issues that you are talking about? This is what occurs at the top of the file:
description: evidence-based annotation of the human genome (GRCh38), version 28 (Ensembl 92)
Should I manually remove this, or where is the tool that you are referring to? Are these issues why the RNA-star job has yet to run on the galaxy (I have no other current jobs running)