Though my data set doesnt have replication, it should run right? but running the cuffdiff shows follwoing error: what can i do for this? Thank you error An error occurred with this dataset: Fatal error: Exit code 139 () [17:25:49] Loading reference annotation. Warning: No conditions are replicated, switching to 'blind' dispersion method [17:26:14] Inspecting maps and determining fragment length distributions. [17:34:12] Modeling fragment co
This can indicate a server-side problem or more commonly an input problem.
First, double check your inputs. This is how: https://galaxyproject.org/support/#troubleshooting
If you cannot find the problem, and are working at Galaxy Main https://usegalaxy.org or can reproduce the problem there, a bug report can be sent in (green bug icon inside the red error dataset). Leave all inputs and outputs undeleted, including any tests you did to confirm proper input content/format. Please also include a link to this Biostars post in the comments.
The alternative is to skip over trying to troubleshoot this problem entirely and instead use the newer RNA-seq methods described in the Galaxy tutorials. Cuffdiff is an older tool and is not really recommended anymore. https://galaxyproject.org/learn/
Thanks! Jen, Galaxy team