Question: Can standard CuffDiff output be used as input into CummeRbund on Galaxy?
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gravatar for connorrogerson
21 months ago by
connorrogerson • 0 wrote:

I've been analysing some RNA-seq data in triplicate and I want to compare replicates by generating scatter plots. This can be done by CummeRbund but I have little experience with R and Galaxy has usually helped me out in these situations. However, the Galaxy version of CummeRbund insists on a SQlite database but I analysed my RNAseq using Cufflinks on my local machine so I didn't have the option of ticking a box for SQlite output. Can CummeRbund on Galaxy function with standard Cuffdiff output? And if so how can this work on Galaxy?

ADD COMMENT • link • modified 21 months ago by Jennifer Hillman Jackson ♦ 25k • written 21 months ago by connorrogerson • 0
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gravatar for Jennifer Hillman Jackson
21 months ago by
United States
Jennifer Hillman Jackson ♦ 25k wrote:

Hello,

The cummeRbund tool as wrapped for Galaxy requires the SQlite input. Perhaps rerun the analysis in Galaxy to generate the proper inputs?

Thanks, Jen, Galaxy team

ADD COMMENT • link written 21 months ago by Jennifer Hillman Jackson ♦ 25k
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