Question: Downloading GFF files from NCBI
gravatar for cjain
15 months ago by
cjain0 wrote:


Can someone help me figure out how to import a genome from the NCBI website into Galaxy in a GFF (or GTF) format? I would like to use HTSeq to quantify our RNA-seq reads onto the downloaded genome.


Chaitanya Jain

rna-seq • 1.0k views
ADD COMMENTlink modified 15 months ago by Jennifer Hillman Jackson24k • written 15 months ago by cjain0
gravatar for Jennifer Hillman Jackson
15 months ago by
United States
Jennifer Hillman Jackson24k wrote:


Refseq annotation files are available for many genomes from NCBI. See:

There are other sources depending on the organism/genome build version. Try UCSC, Ensembl, and the like. Before using with tools, make sure that the reference genome that the annotation is based on is an exact match for the reference genome in use. This includes ensuring that the chromosome identifiers are in the same format.

Best, Jen, Galaxy team

ADD COMMENTlink written 15 months ago by Jennifer Hillman Jackson24k
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