Question: Difference between mRNA, CDS, transcript and gene in annotation file
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divya.nandakumar • 0 wrote:
Hi,
I am new to bioinformatics and have a very basic question. In the annotation files (gff) available in NCBI ftp site, there are different features such as gene, CDS, transcript, mRNA, etc. I understand that CDS represents the coding sequence - i.e. starting from ATG. But I confused between the definitions for gene, transcript and mRNA. If I want to extract 500 bp upstream and downstream from transcription start sites, should I be using the transcript, mRNA or gene?
Thanks! and Sorry about the naivety of the question.
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modified 2.4 years ago
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Jennifer Hillman Jackson ♦ 25k
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2.4 years ago by
divya.nandakumar • 0
