hi i have done qc and then groomed single end sra sequences from ebi. i ran tophat. i am getting error- An error occurred setting the metadata for this dataset Set it manually or retry auto-detection.
though the align summary result says.Reads: Input : 14720816 Mapped : 14253449 (96.8% of input) of these: 1397422 ( 9.8%) have multiple alignments (34022 have >20) 96.8% overall read mapping rate.
please help. thanks