User: juan.perianez

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Posts by juan.perianez

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Comment: C: All reads are 0 in htseq-count
... Hello again, I have one question, I have been looking for the answer but I did not find it. The question is how many reads can you loose in all the process? what is normal? For example I have one sample with 49millions of reads when I used tophat I have 43 (87.5% mapped) and later when I use HTs ...
written 21 months ago by juan.perianez20
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Answer: A: All reads are 0 in htseq-count
... Hello, It is now working! I have the numbers of my first sample! Thanks!! Juan. ...
written 21 months ago by juan.perianez20
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Comment: C: All reads are 0 in htseq-count
... Hi, Thanks for your help, I do not know really how to check this, It is the first time that I use Galaxy. I took the GTF file from the Arabidopsis_thaliana_Ensembl_TAIR10.tar and it present the format that I show below: Seqname Source Feature Start End Score Strand Frame Attributes 1 ensemb ...
written 21 months ago by juan.perianez20
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All reads are 0 in htseq-count
... Hello, I have a trouble using htseq-count, I used TopHat to aling my data and it looks ok but when I tried to quantify the number of reads all the genes present 0 read. You know somethign that I could change to obtain the number of reads? Thanks!! Juan. ...

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