User: selma.maacha

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Posts by selma.maacha

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Allelic sequencing using NGS
... Hello, I am trying to assess indels after CRISPR/Cas9 editing of a gene of interest. I extracted genomic DNA and amplified the locus of interest by PCR (350bp) then submitted the amplicon to NGS. I just got the FASTQ file for the submitted sample (text file with millions of reads, FASTQ format). I w ...
galaxy written 24 months ago by selma.maacha0
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CRISPR/Cas9 and allelic NGS to assess indels homozygocy or heterozygocy
... Hi everyone, I am trying to assess indels after CRISPR/Cas9 editing of a gene of interest. I extracted genomic DNA and amplified the locus of interest by PCR (350bp) then submitted the amplicon to NGS. I just got the FASTQ file for the submitted sample (text file with millions of reads, FASTQ form ...
galaxy written 24 months ago by selma.maacha0 • updated 24 months ago by Martin Čech ♦♦ 4.8k

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