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- 2 years ago
Posts by michael.falta
... Problem solved!! Thank you, thank you, thank you.... The datatype for the assembled reads was fastq. I changed it to fastqsanger, and the barcode splitter worked perfectly. ...
... I ran an Illumina 2 x 250 PER on PCR amplicons and received the data demultiplexed by the Index on Read 2. The read 2 sequences were not quite long enough to reach the UMIs (barcodes) at the end of each sequence, so I then ran PEAR to assemble read 2 and read 1. The output clearly shows the additi ...
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