User: Scott Tighe

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Scott Tighe200
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Posts by Scott Tighe

<prev • 20 results • page 1 of 2 • next >
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Filtering Phix
... Dear Team Galaxy Is there a simple way to filter PhiX control from a sample set? S -- Scott Tighe Senior Core Laboratory Research Staff Advanced Genome Technologies Core NextGen Sequencing/Flow Cytometry University of Vermont and Vermont Cancer Center 149 Beaumont ave Health Science Research Faci ...
galaxy written 3.7 years ago by Scott Tighe200 • updated 3.7 years ago by Jennifer Hillman Jackson23k
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Comment: C: Unsubscribe
... ...
written 3.7 years ago by Scott Tighe200
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Comment: C: Unsubscribe
... Hi Galaxy List If anybody has some insight as to how to upload data to the new Galaxy, I sure would enjoy hearing the trick. Scott Scott Tighe Senior Core Laboratory Research Staff Advanced Genome Technologies Core NextGen Sequencing/Flow Cytometry University of Vermont and Vermont Cancer Center ...
written 3.7 years ago by Scott Tighe200
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Answer: A: Update From Server Failed Several Times
... For all users: The tutorial on uploading data is incorrect. I do not think they have updated "how to upload data" yet. Scott Scott Tighe Senior Core Laboratory Research Staff Advanced Genome Technologies Core NextGen Sequencing/Flow Cytometry University of Vermont and Vermont Cancer Center 149 Be ...
written 3.8 years ago by Scott Tighe200
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Answer: A: Enquiry Regarding The Working Of Galaxy
... Seems to be down a lot these days...Maybe Dave could update us on the future of Galaxy. Scott Tighe Senior Core Laboratory Research Staff Advanced Genome Technologies Core NextGen Sequencing/Flow Cytometry University of Vermont and Vermont Cancer Center 149 Beaumont ave Health Science Research Faci ...
written 3.8 years ago by Scott Tighe200
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Answer: A: Metagenomics Question
... Jing If I have a Galaxy dataset, do you think it is possible to develop a pipeline that can Megablast from Shotgun data for: DNA gyrase only Ribosomal ITSinternal transcribed spacer only Cytochrome RecA Pol As well as filter all model organisms? Have you worked with the Galaxy Toolshed? Scott ...
written 4.2 years ago by Scott Tighe200
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Answer: A: Metagenomics Question
... Jing All good thoughts and if I remember correctly, custom software can indeed to incorparated into Galaxy through use of the "Toolshed" . I'll check into this with Jennifer. Thanks Scott Scott Tighe Senior Core Laboratory Research Staff Advanced Genome Technologies Core University of Vermont V ...
written 4.2 years ago by Scott Tighe200
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Answer: A: Metagenomics Question
... Dear Jing What you have outlined below is perfect. I wonder how hard it would be to design a few filters that only look a certain genes and or filter model organisms out of the dataset. For example, say you want only data for 16s or only gyrase, but no /E.coli/ and no /Pseudomanas aeroginosa/ Sc ...
written 4.2 years ago by Scott Tighe200
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Answer: A: Metagenomics Question
... Jing et al Thank you for the offer to write some code to help advance the metagenomics arena. It is certainly needed. So the problem is well known with megablast and shotgun metagenomics and without proper understanding and correct software will yield very misleading and in many cases incorrect da ...
written 4.2 years ago by Scott Tighe200
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Answer: A: Metagenomics Question
... Gerald 16s is basically useless for identification to genus. Since I started sequencing 16s in 1992, I have come to realize that without sequencing the full 1540 bases, it is generally misleading, and even than, it is not accurate enough to nail genus on more than 1/2 the cases. However, what is ...
written 4.2 years ago by Scott Tighe200

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