User: btarkowski

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btarkowski10
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New User
Location:
Poland
Last seen:
2 years, 4 months ago
Joined:
2 years, 6 months ago
Email:
b*********@iimcb.gov.pl

Posts by btarkowski

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Comment: C: Bowtie2/FreeBayes/mpileup variant detection on NGS of PCR amplicons around Cas9/
... Dear Guy, thanks for your suggestion and sorry for replying with such delay. I'll try to apply those to the next dataset that I'll be analysing in the future. All the best, BartekT ...
written 2.3 years ago by btarkowski10
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Comment: C: Bowtie2/FreeBayes/mpileup variant detection on NGS of PCR amplicons around Cas9/
... Dear Bjorn, thanks for your input and sorry for the delay with my reply. I tried TopHat - with standard setting it maps approx. same fraction of reads (~30%), but maybe one should tweak the run parameters. So far I unfortunately had no time to do this, but I'll keep in mind your suggestion for the ...
written 2.3 years ago by btarkowski10
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Bowtie2/FreeBayes/mpileup variant detection on NGS of PCR amplicons around Cas9/CRISPR indels
... Hey threre, I have an MiSeq experiment using 24 indices where in each index I was sequencing 3 PCR amplified loci (275bp) from triploid clones of mouse cell line that was subjected to Cas9/CRISPR digest, i.e. should contain indels. Moreover clones were not picked very cartefully - sometimes there m ...
freebayes bowtie2 indels cas9 crispr written 2.5 years ago by btarkowski10 • updated 2.5 years ago by Guy Reeves1.0k

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