User: shuo.li
shuo.li • 10
- Reputation:
- 10
- Status:
- New User
- Location:
- Australia
- Last seen:
- 1 year, 7 months ago
- Joined:
- 2 years, 6 months ago
- Email:
- s******@monash.edu
Posts by shuo.li
<prev
• 7 results •
page 1 of 1 •
next >
0
votes
2
answers
376
views
2
answers
Comment:
C: 3-D PCA plot
... Thank you Jen for the reply.
To plot PCA, what type of data do I need?
I uploaded the RNAseq data that I got from service provider, but have not
yet trimmed or aligned to human genome. Do I need to do this two things
first?
Thanks a lot for your help
Lucy ...
written 19 months ago by
shuo.li • 10
0
votes
2
answers
376
views
2
answers
... Hi,
does anyone know if Galaxy.org can do principle component analysis? my boss asked for a 3-D PCA figure and I have no clue how to do it.
Thanks for any tip on this,
Lucy
...
written 19 months ago by
shuo.li • 10
• updated
19 months ago by
y.hoogstrate • 460
1
vote
1
answer
367
views
1
answer
... Hi,
Could an expert please give me a lay instruction on below matters? --
Q1: I have uploaded my RNAseq data (gene expresssion) to galaxy, what is the next step? such as quality control??
Q2: I have used up 95% of my space, is there any possiblity to get more space, such as create a 2nd account ...
written 20 months ago by
shuo.li • 10
• updated
20 months ago by
Jennifer Hillman Jackson ♦ 24k
2
votes
1
answer
510
views
1
answer
... Hi,
My RNAseq data are huge, ~ 3 to 5 GB each. The university service provider ftp side has password, I asked but they say it is not possible to remove the password.
Thus not possible for Galaxy to fetch it from the ftp side.
Is there another way? I heard about the "library" upload, but could n ...
written 21 months ago by
shuo.li • 10
• updated
21 months ago by
Jennifer Hillman Jackson ♦ 24k
1
vote
1
answer
966
views
1
answer
... Dear colleagues,
It is my first RNAseq project, and I am a lab scientist with no expertise in bioinformatics.
I received RNAseq data from our NGS service provider, they are in .fastq.gz.
Q: can I upload this .gz format to galaxy or need to first convert to decompress the files?
Thanks a lo ...
written 21 months ago by
shuo.li • 10
• updated
21 months ago by
Martin Čech ♦♦ 4.5k
0
votes
1
answer
857
views
1
answers
... Thank you very much for the helpful explanation.
I have another question: after quality filter some reads become
considerable shorter. If I want to filter out all reads <50nt, or keep all
the reads > 50nt, for a fastq file, how to do this?
Thanks a lot for your help
Shuo Li ...
written 2.6 years ago by
shuo.li • 10
0
votes
1
answer
857
views
1
answer
... Dear Galaxy experts,
I need to merge R1 and R2 Illumina paired end reads. The amplicon was ~550nt, sequenced using 300nt x2 Illumina chemistry. The 2 reads should overlap in middle. so it is an align/merge task.
After loading the data to Galaxy, I could not figure out what to ...
written 2.6 years ago by
shuo.li • 10
• updated
2.6 years ago by
Jennifer Hillman Jackson ♦ 24k
Latest awards to shuo.li
No awards yet. Soon to come :-)
Use of this site constitutes acceptance of our User
Agreement
and Privacy
Policy.
Powered by Biostar
version 16.09
Traffic: 153 users visited in the last hour